Titlebook: Cardiovascular Proteomics; Methods and Protocol Fernando Vivanco Book 2007 Humana Press 2007 Proteomics.Translation.Vivo.bioinformatics.car

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Springer Proceedings in Physicsradients to separate the sample proteins, combined with quantitative computer analysis to detect differentially expressed proteins and mass spectrometry technologies to identify proteins of interest. In spite of the development of novel gel-free technologies, 2DE remains the only technique that rout

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Some Extensions of the Lucas Functions,anisms still remain unclear. In order to identify proteins that are involved in ischemia-reperfusion injury, we compared precipitated 100,000. fractions of normal, ischemic, and ischemic-reperfused rat hearts using two-dimensional (2D) difference gel electrophoresis (2D-DIGE). 2D-DIGE is reliable me

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Jan C. A. Boeyens,Demetrius C. Levendispertension is associated with such adverse effects as accelerated arteriosclerosis and pathological left ventricular hypertrophy, among others. The molecular mechanisms affecting left ventricular hypertrophy remain mostly unknown. The advent of proteome profiling has facilitated the elucidation of d

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https://doi.org/10.1007/978-1-4020-6660-3raction of proteins from tissue samples should be designed to maintain a limited dynamic range in the concentration of proteins present. However, in many tissue extracts a high concentration of serum albumin exists from tissue perfusion and/or an inability to effectively rinse the tissue owing to su

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Jan C. A. Boeyens,Demetrius C. Levendises in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic/ intracellular context, has become the most commonly used preparative/enrichment method. This ch

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