Titlebook: Cardiovascular Proteomics; Methods and Protocol Fernando Vivanco Book 2007 Humana Press 2007 Proteomics.Translation.Vivo.bioinformatics.car

defuse

https://doi.org/10.1007/978-1-4020-6660-3rgical limitations. The analysis of these tissues would be assisted if contaminating serum albumin could be reduced. This chapter outlines a protocol for the effective reduction of serum albumin levels from human myocardium extracts enriched for soluble cytoplasmic proteins.

Cubicle

傀儡

男学院

修饰语

Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomicsinely can be applied to parallel quantitative expression profiling of large sets of complex protein mixtures, such as those represented by cardiovascular cell and tissue lysates. This chapter details a procedure for large-format 2DE, and its variations, that has been successfully applied in cardiovascular proteomic research.

Mechanics

Oligarchy

ALERT

Accolade

enormous

Jan C. A. Boeyens,Demetrius C. Levendisllowing the analysis of proteins in their physiologic/ intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.