Titlebook: Cardiovascular Development; Methods and Protocol Xu Peng,Warren E. Zimmer Book 2021Latest edition Springer Science+Business Media, LLC, par

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Preparation of Neonatal Rat Papillary Muscles for Contractile Studies,impossible to perform in vivo. In contrast to ventricular muscle strip preparations, papillary muscles can be prepared without severely damaging the muscle tissue. In this preparation, the isolated papillary muscle is fixed in an environmentally controlled organ bath chamber and electrically stimula

AUGUR

In Vitro Study of Permeability in Lymphatic Endothelial Cells Responding to Histamine,. The increased permeability impairs the barrier function of vessels to fluid, soluble electrolytes, and proteins, resulting in the swelling of interstitial tissues, termed edema and lymphedema. Here, we describe two approaches to study the permeability of LECs, to macromolecules or to electrolytes,

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Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent-Derived Stem Cells,el systems with which to investigate mechanisms of disease and to develop and test potential therapies. The use of human-derived induced pluripotent stem cells (iPSCs) differentiated into cardiomyocytes aims to address this need. While cardiac differentiation protocols have been established previous

Offbeat

Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining,lood vessels results in embryonic lethality and also contributes to the pathogenesis of a number of human diseases, including cancer metastasis. To understand the molecular events associated with blood vessel formation, we established a fluorescence staining-based protocol on mouse embryonic skin. W

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Detecting Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain,any diseases in the adult, including inflammatory, ischemic, and cancer metastatic diseases. Brain contains abundant blood vessels and has some unique physiological functions, such as blood-brain barrier. Due to the thickness and opaque characters of the tissues, it is a challenge to visualize the t

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Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy,nimal photodamage and photobleaching as compared to traditional confocal microscopy. As this technology becomes much more readily available, it will be useful in revealing new findings in the cardiovascular development field that may be hidden or difficult to image. In this manuscript, we describe a