Titlebook: Cardiovascular Development; Methods and Protocol Xu Peng,Warren E. Zimmer Book 2021Latest edition Springer Science+Business Media, LLC, par

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Isolation of Lymphatic Muscle Cells (LMCs ) from Rat Mesentery, the spontaneous contraction of the lymphatic vessels to pump fluid forward. However, our understanding of the more detailed molecular phenotypes of LMCs is limited. Here, we described a method to isolate the LMCs from rat mesentery and then culture the cells in vitro, which will serve a lot more mo

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Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus,myocyte contractility, heart hypertrophy, and cardiac failure. In contrast to neonatal cardiomyocyte isolation, adult mice cardiomyocytes isolation is challenging due to firm connections among cardiomyocytes through intercalated discs. The availability of newly generated genetically modified mouse l

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Nuclear Methods in Mineralogy and Geologymine vascular ECs-specific genes expression in vivo. Here, we described a method to dissect vascular ECs from the frozen heart tissue by LCM. The total RNA or proteins are then extracted from the ECs for further analysis.