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Titlebook: Carbohydrate-Based Vaccines; Methods and Protocol Bernd Lepenies Book 2015 Springer Science+Business Media New York 2015 AutoMap software.C

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Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods, This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-M
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Glycoconjugate Vaccines: The Regulatory Framework,f a prospective vaccine is a key prerequisite for approval. Undesired side effects would not only have the potential to damage the individual infant but also lead to a loss of confidence in the respective vaccine—or vaccines in general—on a population level. Thus, regulatory requirements, particular
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Families of Measures and their Envelopes, raise oligosaccharide-specific antibodies in mice, and splenocytes are fused with myeloma cells to form hybridomas. Hybridoma clones producing oligosaccharide-specific mAbs are selected by glycan microarray screening. Selected clones are expanded and mAbs are purified from the cell culture supernat
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Carbohydrate-Based Vaccines: An Overview,ment of carbohydrate-based vaccines and led to the approval of CPS-based vaccines against ., . type b (Hib), and .. Meanwhile, it was observed that CPS generally do not elicit protective antibody responses in children below the age of 2 years who are at the greatest risk of infection. As a consequen
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