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Titlebook: Carbohydrate-Based Vaccines; Methods and Protocol Bernd Lepenies Book 2015 Springer Science+Business Media New York 2015 AutoMap software.C

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发表于 2025-3-21 19:11:17 | 显示全部楼层 |阅读模式
书目名称Carbohydrate-Based Vaccines
副标题Methods and Protocol
编辑Bernd Lepenies
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Carbohydrate-Based Vaccines; Methods and Protocol Bernd Lepenies Book 2015 Springer Science+Business Media New York 2015 AutoMap software.C
描述.This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Carbohydrate-Based Vaccines: Methods and Protocols. aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines..
出版日期Book 2015
关键词AutoMap software; Carbohydrates; carbohydrate antigens; glycan microarray fabrication; glycans; glycoconj
版次1
doihttps://doi.org/10.1007/978-1-4939-2874-3
isbn_softcover978-1-4939-4919-9
isbn_ebook978-1-4939-2874-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens,es by mass spectrometry is difficult, and detection reagents, such as monoclonal antibodies and lectins, are only available for a small subset of glycans. An alternative approach involves profiling serum anti-glycan antibody populations to identify unique antibodies or changes in antibody subpopulat
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Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens, is a frequent feature of cancer cells. Antibodies recognizing such carbohydrate antigens may be used for the specific detection of potentially harmful cells, immunohistochemistry, and diagnostic and therapeutic applications. The generation of specific and strongly binding antibodies against defined
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Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Enc) are preferred to ELISA titers for evaluating protection against infection. Such assays are normally performed using phagocytic cell lines or purified cell types, which underestimate the complexity of blood bactericidal activity. Here, we describe an OPA using murine whole-blood as effector cells,
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Determination of ,-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry anscribe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.
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Temporary Conversion of Protein Amino Groups to Azides: A Synthetic Strategy for Glycoconjugate Vacto screen lectins, toxins, and antibodies. A novel approach to potentiate and profile the immune response to vaccines involves targeting antigens directly to dendritic cells (DCs), the key cells engaged in the immunization process. Inclusion of a carbohydrate ligand recognized by C-type lectins expr
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