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Titlebook: Capturing Chromosome Conformation; Methods and Protocol Beatrice Bodega,Chiara Lanzuolo Book 2021 Springer Science+Business Media, LLC, par

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书目名称Capturing Chromosome Conformation
副标题Methods and Protocol
编辑Beatrice Bodega,Chiara Lanzuolo
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Capturing Chromosome Conformation; Methods and Protocol Beatrice Bodega,Chiara Lanzuolo Book 2021 Springer Science+Business Media, LLC, par
描述This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Capturing Chromosome Conformation: Methods and Protocols. serves as an ideal guide for researchers working to further understand 3D genome organization..
出版日期Book 2021
关键词3D genome organization; Tridimensional chromatin conformation; Chromosome conformation capture; Nuclear
版次1
doihttps://doi.org/10.1007/978-1-0716-0664-3
isbn_softcover978-1-0716-0666-7
isbn_ebook978-1-0716-0664-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Jos Dute,Michael G. Faure,Helmut Koziolth CRISPR/Cas9 gene editing, to discreetly label genes for live cell imaging to study chromatin dynamics. Our approach improves on some of the fundamental shortfalls associated with current labelling strategies and has the potential for multiplexed observations.
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Single-Cell DamID to Capture Contacts Between DNA and the Nuclear Lamina in Individual Mammalian Cena protein lamin B1, genomic regions in contact with the nuclear periphery can be mapped. Single-cell DamID generates contact maps with sufficient throughput and resolution to reliably identify patterns of similarity as well as variation in nuclear organization of interphase chromosomes.
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Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHth CRISPR/Cas9 gene editing, to discreetly label genes for live cell imaging to study chromatin dynamics. Our approach improves on some of the fundamental shortfalls associated with current labelling strategies and has the potential for multiplexed observations.
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1064-3745 expertsThis detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the h
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