Titlebook: Capturing Chromosome Conformation; Methods and Protocol Beatrice Bodega,Chiara Lanzuolo Book 2021 Springer Science+Business Media, LLC, par

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,3D COMBO chrRNA–DNA–ImmunoFISH,ured via the concerted action of different players (transcription factors, “reader” and “writer” enzymes, histone marks, structural proteins, noncoding regulatory RNAs) that flow in the 3D organization of the genome. Indeed, nuclear architecture participates in the punctual and cell-type-specific re

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Targeted DNase Hi-C,g phenotype-associated chromatin 3D signatures. Here, I describe a detailed protocol of targeted DNase Hi-C library preparation, which covers experimental steps starting from cell cross-linking to library amplification.

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Formaldehyde-Mediated Snapshot of Nuclear Architecture,hogens..Experimental treatments often require the isolation of cells from their physiological environment, exposing them to possible sudden changes in their nuclear architecture. Here we propose an early cross-linking on primary cells, a fixing method that can help to minimize the risk of nuclear st

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