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Titlebook: Capillary Electrophoresis of Nucleic Acids; Keith R. Mitchelson,Jing Cheng Book 2001 Humana Press 2001 DNA.Nucleotide.PCR.RNA.fluorescence

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Toward Effective PCR-Based Amplification of DNA on Microfabricated Chipsdetecting infectious agents. Primers, short pieces of DNA complementary to the DNA sequence of interest, are mixed with nucleotides, a small amount of template DNA from the sample of interest, and . DNA polymerase enzyme in the appropriate buffer. Using temperature cycling, a short piece of DNA (50-
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Low-Stringency Single Specific Primer-Amplified DNA Analysis by Capillary Electrophoresistity testing. Most PCR-based typing assays that discriminate single nucleotide polymorphisms (SNPs) require further manipulation of the amplification product to identify the polymorphic sites. A more simplified assay, PCR products would be directly analyzed to determine whether DNA extracted from tw
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https://doi.org/10.1007/978-1-4842-1329-2time per sample. This has been accomplished by using a range of an effective length capillary as short as 7 cm and field strength as high as 2000 V/cm for the separations of small ions, drugs, and proteins (.-.).
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Merge Algorithms for Intelligent Vehicles, DNA molecules caused when the restriction enzymes do their cutting are called restriction fragment-length polymorphisms (RFLP). Another molecular procedure that allows one to work with small amounts of DNA is the polymerase chain reaction (PCR), a method for amplifying and enriching the sample with
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Sumitra Mallick,Mrutyunjaya Pandaradiolabeled (or using radiolabeled primers). Then following nondenaturing gel electrophoresis, the mobility (position) of the ssDNA is detected by autoradiography (.-.). In spite of the simplicity of the technique, conventional SSCP analysis remains both labor-intensive and time-consuming, involvin
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