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Titlebook: Capillary Electrophoresis of Nucleic Acids; Keith R. Mitchelson,Jing Cheng Book 2001 Humana Press 2001 DNA.Nucleotide.PCR.RNA.fluorescence

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发表于 2025-3-21 16:37:47 | 显示全部楼层 |阅读模式
书目名称Capillary Electrophoresis of Nucleic Acids
编辑Keith R. Mitchelson,Jing Cheng
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Capillary Electrophoresis of Nucleic Acids;  Keith R. Mitchelson,Jing Cheng Book 2001 Humana Press 2001 DNA.Nucleotide.PCR.RNA.fluorescence
描述The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the i
出版日期Book 2001
关键词DNA; Nucleotide; PCR; RNA; fluorescence; gene expression; temperature; tissue
版次1
doihttps://doi.org/10.1385/1592591167
isbn_softcover978-1-61737-177-6
isbn_ebook978-1-59259-116-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2001
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发表于 2025-3-21 23:39:51 | 显示全部楼层
Ultra-Fast DNA Separations Using Capillary Electrophoresisze selective separations using synthetic polyacrylamide sieving gels (SDS-PAGE) (.), development of the two dimensional electrophoresis (2D-PAGE) (.) together with the use of the polyacrylamide gels for DNA sequencing (., .) revolutionized the field of bio-separations (.-.). At present, slab-gel ele
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Fast DNA Fragment Sizing in a Short Capillary Columnnely used for the separation of double-stranded DNA fragments; single-stranded DNA fragments and polymerase chain reaction (PCR) products in our laboratory. CE is a versatile tool for separating nucleic acids in molecular biology (.). CE has the advantages of automation, small sample requirement, fa
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Detection of DNA Polymorphisms Using PCR-RFLP and Capillary Electrophoresiscleotide polymorphism or SNP, can occur approximately once every several hundred base pairs. The ability to accurately determine the DNA sequence at specific sites throughout an organism’s genome is important in a variety of applications including disease detection, differentiation of microorganisms
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High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE)er research, and molecular diagnostics. The large arsenal of methods for mutation detection ranges from direct sequencing and array hybridization to allele-specific polymerase chain reaction (PCR). CDCE (.) holds a unique position within this list of techniques due to its high flexibility. Having se
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Point Mutation Detection by Temperature-Programmed Capillary Electrophoresiss, Alzheimer disease, Duchenne muscular dystrophy, and various thalassemias. These alterations in DNA sequence include many types of mutations and polymorphisms, such as substitutions of one or several nucleotides, deletions or insertions of some larger sequences, differences in variable number of t
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Amplification Refractory Mutation System Analysis of Point Mutations by Capillary Electrophoresistions in human diseases. DNA technologies allow great advances in basic knowledge of the pathophysiology of disorders, adding new means of diagnosis to characterize the molecular defect and to correlate between genotype and phenotype. The field of molecular diagnosis is evolving rapidly, and current
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SSCP Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detectorhod for the screening of unknown mutation in small stretches of DNA. Its widespread use is related to its simplicity and low cost. SSCP analysis is based on the principle that ssDNA with a single base substitution (or containing a small sequence deletion or insertion) often assumes an altered folded
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