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Titlebook: Capillary Electrophoresis of Nucleic Acids; Keith R. Mitchelson,Jing Cheng Book 2001 Humana Press 2001 DNA.Nucleotide.PCR.RNA.fluorescence

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楼主: 一个希拉里
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The Natural Language Modeling Procedurection. However, these tests require three separate PCR amplifications to be run using conventional methods. The use of multicolor fluorescence detection and CAE can simplify this improved diagnostic test.
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High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE) identifying mutants with precision comparable to that of direct sequencing (.). In addition, the instrument for CDCE does not need to be dedicated to one purpose. In fact, most capillary DNA electrophoresis systems, such as DNA sequencing capillary instruments, can be adapted for CDCE separations.
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Single-Nucleotide Primer Extension Assay by Capillary Electrophoresis Laser-Induced Fluorescence applications of CE-LIF is the analysis of DNA either by specifically labeling one or more nucleotides with a fluorescent tag (e.g., on the primer or the dideoxyterminator) or nonspecifically detecting DNA through intercalation or similar binding of dyes (.).
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SSCP Analysis of Point Mutations by Multicolor Capillary Electrophoresisseful, for example in surveying for possible mutations in genes suspected to be responsible for genetic diseases, or finding single-nucleotide polymorphisms (SNPs) in blindly amplified genomic segments.
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The End of Disk? SSD and In-Memory Databasesonly low electric field strength can be applied without excessive Joule heating. Numerous efforts to increase the separation speed have been taken, typically applying very thin gel slabs, allowing higher electric field strength during the separation.
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Asier Alonso Tejeda,Pablo Prieto Arceimportant applications in the fields of genetic and medical research, clinical chemistry and forensic science. Because a large portion of sequence variations in the human genome is caused by single base changes, any method used to detect mutations or polymorphisms must be capable of detecting single-base substitutions.
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Martin Strelec,Pavel Hering,Per Janecekly, it broadly comprises genetic disease analysis, paternity testing, forensic studies, assessment of genetic risk, and so on. A number of molecular techniques have been developed during the last 10–15 yr that greatly improve diagnostic and prognostic capabilities, allowing carrier detection, and prenatal diagnosis.
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