Titlebook: Candida Species; Methods and Protocol Richard Calderone Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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The Techniques Innovated to Support NSE,ologic response and resulting symptomatic condition and focuses further on assays used to demonstrate “neutrophil anergy” in the model. These methods may serve as a source or resource for further experimentation with the ultimate goal to reduce or eliminate VVC/RVVC.

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Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic An1000783, 2009). We also outline the steps to confirming the genotype of the resulting transformants as well as an example of the use of double heterozygous deletion mutants for complex haploinsufficiency analysis of biofilm formation.

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Using Synthetic Genetic Interactions in , as a Novel Method to Detect Genes with Roles in Antifungalcan aid in bridging our understanding of complex genetic networks, with applications to diverse microbial pathogens to further our understanding of virulence, the use of antimicrobial therapies, and host–pathogen interactions.

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Genetic Screens of an Anti-, Natural Product Using the Heterozygous , Mutant Librarya direct link between a phenotypic screen (easy read-out) and the identity of the gene target. Screens using mutant libraries can identify chemical–genetic interactions and genes or pathways affected by compounds to decipher the mechanism of action. Herein, we describe a genetic screen of an anti-. natural product.

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Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anerologic response and resulting symptomatic condition and focuses further on assays used to demonstrate “neutrophil anergy” in the model. These methods may serve as a source or resource for further experimentation with the ultimate goal to reduce or eliminate VVC/RVVC.

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