Titlebook: Candida Species; Methods and Protocol Richard Calderone Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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High-Throughput Phospholipidomics of , Cells: From Sample Preparation to Data Analysislatform, we can achieve rapid, sensitive, and accurate quantification of lipids in . extracts. Moreover, the approach is simple and relatively cost-effective. Below, we discuss the key features of ion trap HPLC-ESI-MS/MS-based comparative lipidomics of . cells.

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The Techniques Innovated to Support NSE,ixed with formaldehyde in whole cells which are then lysed; (2) the fixed chromatin is digested with a carefully chosen restriction enzymes to separate intervening DNA fragments; (3) the fragments brought into proximity by DNA looping are ligated in conditions favoring intramolecular ligation; and (