Titlebook: Candida Species; Methods and Protocol Richard Calderone,Ronald Cihlar Book 2016 Springer Science+Business Media New York 2016 C. albicans.a

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Biofilms and Antifungal Susceptibility Testingred biofilm structure. In this chapter we describe the most widely used assays for the determination of biofilm production and assessment of susceptibility of biofilms to antifungal agents or host phagocytes as various methods, the most frequent of which are staining, confocal laser scanning microsc

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Typing , Species Using Microsatellite Length Polymorphism and Multilocus Sequence Typingues have been developed. Two methods have emerged in the 2000s as soon as enough knowledge of the . spp. genomes was available to choose adequate loci and primers, namely microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST). To contrast with previous PCR-based methods, spec

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Diagnostic Methods for Detection of Blood-Borne Candidiasisndidemia and other types of invasive candidiasis, but their clinical roles are unclear. In this chapter, we describe laboratory protocols for performing Fungitell and T2Candida assays. We then discuss step-by-step methods for interpreting test results at the bedside using a Bayesian framework, and f

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1064-3745 alls..Authoritative and practical, .Candida Species: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-4939-7980-6978-1-4939-3052-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Jens Schneider,Maurice Crul,Andreas Potts suitable for detecting cytoplasmic protein interactions in their natural subcellular environment, and has been successfully used to investigate protein-protein interactions, including transcription factor associations, in ..

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The Fungal Biome of the Oral Cavitycterizing the oral mycobiome (the fungal component of the oral microbiota). Our group recently characterized the oral mycobiome in health and disease focusing on HIV. In this chapter we will describe the methods used by our group for characterization of the oral mycobiome.

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How to Use the , Genome Database searched, retrieved, and analyzed. Starting with the guided tour of the CGD Home page and Locus Summary page, this unit shows how to navigate the various assemblies of the . genome, how to use Gene Ontology tools to make sense of large-scale data, and how to access the microarray data archived at CGD.

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Chromatin Immunoprecipitation (ChIP) Assay in ,omic loci in cellular context can be determined by this technique. Here, we are describing a ChIP protocol that is optimized for .. The protocol requires 4–5 days for completion of the assay and has been used to produce robust ChIP results for diverse proteins in this organism and its related species including . and ..