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Titlebook: Candida Species; Methods and Protocol Richard Calderone,Ronald Cihlar Book 2016 Springer Science+Business Media New York 2016 C. albicans.a

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Chromatin Immunoprecipitation (ChIP) Assay in ,locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. The end result of this process is the enrichment of DNA fragments associated with the desired protein. Thus, interactions between proteins and gen
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Assessing Mitochondrial Functions in ,de drop plate assays for evaluating inhibitors of the respiratory electron transport system complexes as well as measuring the enzyme activity of complex I-V enzyme activities. Assays are also presented to measure toxic ROS production that accompanies gene mutations or gene loss and chronological ag
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Imaging Candida Infections in the Hoste a primary target organ of ., and invasion into the kidney medulla can lead to loss of renal function and death. Therefore, development of noninvasive methods to assess kidney infections could aid in the management of disseminated candidemia. We describe a magnetic resonance imaging method utilizin
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Interaction Models: Antibiotic Resistance Testing and Host Interactionsequences for virulence. Hyphae-associated . is less susceptible to antibiotic treatment. Furthermore, co-inoculation of . and . causes more severe and widespread infection than either microorganism alone. In this chapter, a basic . model for studying the interaction between . hyphae and . is present
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Genetic Screens for Determination of Mechanism of Actionntinued progress of the compound through the pipeline. There are many approaches that can be utilized in understanding the MOA. One of these approaches is a genetic screen utilizing the availability of . mutant libraries. Both null and heterozygous library mutants covering the entire genome of this
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