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Titlebook: Calcium Signaling Protocols; David G. Lambert Book 19991st edition Springer Science+Business Media New York 1999

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Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilr of protein kinase C isozymes, Ins(1,4,5)P. mobilizes intracellular Ca.. G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLCγ isozymes (.). Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P..
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Helmuth Harig,Christian J. Langenbach [Ca.]., but a revolution in the study of intracellular [Ca.]. occurred when fluorescent dyes for Ca. were developed (.). Since then, fluorescent dyes and fluorescent microscopy have been used to observe resting and nonresting [Ca.] in intact cells as well as in subcellular fractions.
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Neue Materialien — Trends und Entwicklungen current knowledge of cellular calcium homeostasis (.). Typically, the fluorescent signal has been monitored using cuvet-based fluorometers or by confocal fluorescent microscopy. Although acceptable for a number of applications, these methods are relatively labor intensive and are not suitable for screening large numbers of compounds.
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G. Günter Voß,Werner Holly,Klaus Boehnker of protein kinase C isozymes, Ins(1,4,5)P. mobilizes intracellular Ca.. G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLCγ isozymes (.). Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P..
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Realitätsbezüge im Mathematikunterrichttion equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or she can move toward developing a protocol that will suit the cells, the experimental objectives, and the equipment available to the investigator.
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Gerhard Strube,Christoph Hölscher and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransmitter release (.). In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca. channels to reduce Ca. influx (.,.).
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Fluorescent Measurement of [Ca2+]ction equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or she can move toward developing a protocol that will suit the cells, the experimental objectives, and the equipment available to the investigator.
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