Titlebook: Calcium Signaling Protocols; David G. Lambert Book 2005Latest edition Humana Press 2005 ATP.ATPase.Calcium.Endoplasmatisches Reticulum.Nuc

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Measurement of [Ca2+]i in Cell Suspensions Using Indo-1mulation of receptors, belonging to almost all the classes so far described, will result in changes in [Ca.].. These changes might be directly induced by either Ca.-influx or Ca.-mobilization from intracellular stores, or indirectly by a number of other mechanisms (.–.). The development of fluoresce

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Confocal Microscopyhow recent advances in this technology, allied with the continued development of Ca.-sensitive fluorescent probes, have provided us with methodologies for unravelling the complexities of Ca. signaling at the cellular and subcellular level. Specifically, we will provide a detailed methodology for the

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Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentrationr cell parameters, but the usefulness of this approach is limited by several problems, not the least of which is temporal averaging. In population studies, agonists often evoke a characteristic peak and plateau type of response irrespective of the stimulus intensity. However, equivalent experiments

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Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer measurements of intracellular Ca. concentration (.Chapter 7), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications, the primary limitation of FLIPR (i.e., its requirement for single wavelength fluorescent probes using vis

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Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocog inositol phospholipid-specific phospholipase C (PLC). Activated PLC catalyzes the hydrolysis of phosphatidylinositol bisphosphate (PIP.), a minor membrane phospholipid, to form two second messengers, diacylglycerol (DAG) and inositol (1,4,5)trisphosphate [Ins(1,4,5,)P.]. DAG is a direct activator

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Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assayiverse cellular processes including neuronal signaling, smooth muscle contraction, fertilization, and sensory perception (.). Ins(1,4,5)P. formation is triggered by the activation of a wide variety of seven-transmembrane, G protein-linked receptors, e.g., muscarinic, glutamate, dopamine, and opioid