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Titlebook: Calcium Signaling Protocols; David G. Lambert Book 2005Latest edition Humana Press 2005 ATP.ATPase.Calcium.Endoplasmatisches Reticulum.Nuc

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书目名称Calcium Signaling Protocols
编辑David G. Lambert
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Calcium Signaling Protocols;  David G. Lambert Book 2005Latest edition Humana Press 2005 ATP.ATPase.Calcium.Endoplasmatisches Reticulum.Nuc
描述In the first edition of Calcium Signaling Protocols I began by writing “The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for
出版日期Book 2005Latest edition
关键词ATP; ATPase; Calcium; Endoplasmatisches Reticulum; Nucleotide; molecular biology; protein; reproducible tec
版次2
doihttps://doi.org/10.1385/1592599494
isbn_softcover978-1-61737-573-6
isbn_ebook978-1-59259-949-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.), however, these preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to cells. The author will describe a .Ca.-release assay u
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Microinjection of ,-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adhanalogs, such as photolabile “caged Ins(1,4,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.); however, microsomal preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to permea
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1064-3745 ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for978-1-61737-573-6978-1-59259-949-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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,Haltestellenplanung in Städten,analogs, such as photolabile “caged Ins(1,4,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.); however, microsomal preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to permea
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