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Titlebook: Calcium Signaling Protocols; David G. Lambert Book 2005Latest edition Humana Press 2005 ATP.ATPase.Calcium.Endoplasmatisches Reticulum.Nuc

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Measurement of [Ca2+]i in Cell Suspensions Using Indo-1ally dyes that respond to Ca. with shifts of excitation or emission spectra (.–.). By measuring at two selected wavelengths (either dual emission or dual excitation), it is possible to calculate the proportion of dye in the Ca.-bound and Ca.-free forms.
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1064-3745 r Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! T
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Ulfert Herlyn,Gitta Scheller,Wulf Tessinrange of detection equipment that can be used. Consequently, this chapter is designed to provide the user with an overview of the technology in order that he or she can move toward developing a protocol that will suit the experimental objectives, the cells, and the equipment available to the investigator.
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Studies in Contemporary Economicsnd messenger. Through this relationship, activation of VSCCs is tightly coupled to the gamut of cellular functions dependent on intracellular Ca., including muscle contraction, energy metabolism, gene expression, and exocytotic/endocytotic cycling.
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Measurement of [3H]PN200-110 and [125I]ω-Conotoxin MVIIA Binding and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransmitter release (.). In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca2. channels to reduce Ca. influx (.,.).
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