Titlebook: CRISPR; Methods and Protocol Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fi Book 2015 Springer Science+Business Media New York 2015 CRI

方面

书目名称CRISPR影响因子(影响力)




书目名称CRISPR影响因子(影响力)学科排名




书目名称CRISPR网络公开度




书目名称CRISPR网络公开度学科排名




书目名称CRISPR被引频次




书目名称CRISPR被引频次学科排名




书目名称CRISPR年度引用




书目名称CRISPR年度引用学科排名




书目名称CRISPR读者反馈




书目名称CRISPR读者反馈学科排名

misanthrope

Musket

GRAZE

休战

Samiran Bera,Santosh Kumar Das,Joydev Ghoshs a metal-dependent endonuclease activity. Here we describe the protocols for the analysis of nuclease activity of purified Cas1 proteins against various DNA substrates including Holliday junctions and other intermediates of DNA recombination and repair.

吊胃口

吊胃口

In Vitro Co-reconstitution of Cas Protein Complexes,roduced as inactive inclusion bodies. Here, we present a detailed protocol for the isolation and purification of insoluble Cas proteins. Guidelines for their solubilization via co-reconstitution strategies and procedures to upscale the production of soluble multimeric Cas protein complexes are provided.

种属关系

Rapid Multiplex Creation of , Strains Capable of Interfering with Phage Infection Through CRISPR,,” can be used for very simple and rapid construction of multiple . strains capable of targeting, through CRISPR interference, any phage or plasmid of interest. Availability of such strains should allow rapid progress in the analysis of CRISPR-Cas system function against diverse mobile genetic elements.

暗语

Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes,es of crRNA-Cas complexes can be quantified by calculating the dissociation constant (..). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from . as an example.

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