Titlebook: Breast Cancer; Methods and Protocol Jian Cao Book 2016 Springer Science+Business Media New York 2016 diagnosing breast cancer.clinical prac

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Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)s can be quantified by this approach. It is well known that changes in mRNA expression in disease, and correlation of mRNA expression profiles with clinical parameters, serve as clinically relevant biomarkers. Hence, accurate determination of the mRNA levels is critically important in describing the

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Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancerde of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA s

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Pyrosequencing Analysis for Breast Cancer DNA Methylomesamples, it is very difficult to get methylation profile of only tumor cells. Laser capture Microdissection (LCM) is giving us a chance to isolate the DNA only from the tumor cells without any stroma cell’s DNA contamination. Once we capture the breast tumor cells, we can isolate the genomic DNA whi

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Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cesitivity testing of short-term cultures of CTCs, single cell sorting of CTCs by fluorescence activated cell sorting (FACS), animal injection tumor and/or metastasis formation studies, next generation sequencing (NGS), gene expression profiling, gene copy number determination, and epigenomic analyses

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Cellular Apoptosis Assay of Breast Cancery introduce methodologies to assay caspases dependent biochemical and morphological changes in vitro breast cancer cell lines and in vivo breast cancer tissues. In addition, mitochondrial involvement is crucial to distinguish two different apoptotic pathways. Methodology to assay dissipation of mito

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Assessment of Matrix Metalloproteinases by Gelatin Zymographyto breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression. In this approach, enzymes are resolved electrophoretically on a sodium dodecyl sulfate-polyacrylamide gel copolymerized with the substrate for the MMP of interest. Post electrophoresis, t

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Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate AssayM). While MMPs are involved in many normal biological processes, unregulated MMP activity has been linked to many detrimental diseases, including cancer, neurodegenerative diseases, stroke, and cardiovascular disease. Developed as tools to investigate MMP function and as potential new therapeutics,