Titlebook: Breast Cancer; Methods and Protocol Jian Cao Book 2016 Springer Science+Business Media New York 2016 diagnosing breast cancer.clinical prac

deep-sleep

书目名称Breast Cancer影响因子(影响力)




书目名称Breast Cancer影响因子(影响力)学科排名




书目名称Breast Cancer网络公开度




书目名称Breast Cancer网络公开度学科排名




书目名称Breast Cancer被引频次




书目名称Breast Cancer被引频次学科排名




书目名称Breast Cancer年度引用




书目名称Breast Cancer年度引用学科排名




书目名称Breast Cancer读者反馈




书目名称Breast Cancer读者反馈学科排名

biopsy

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Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.

PARA

催眠

Clinical Applications for Immunohistochemistry of Breast Lesionsies to the best course of clinical action. In this chapter, we discuss the use of immunohistochemistry testing for some of the key molecular markers involved in breast pathology that are crucial for classifying breast cancers and the guidelines for the interpretation of testing results that assist in clinical management.

maintenance

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MOTIF

Breast Cancer Stem Cell Isolationhus, the technique to identify and purify the cancer stem cell is the key in any cancer stem cell research. In this protocol, we provide the basic technology of identification and purification of breast cancer stem cells as well as further functional assays to help the researchers achieve their research goals.

Perennial长期的

Cellular Apoptosis Assay of Breast Cancerchondrial transmembrane potential, an early event of mitochondrial involvement, is also included. Of note, since apoptotic features may not appear to the same extent depending on the context of cell types and the death-inducing insults, a common practice is to use more than one method to assess apoptosis, qualitatively and quantitatively.

LINES

Assessment of Matrix Metalloproteinases by Gelatin Zymographyhe enzymes are refolded in order for proteolysis of the incorporated substrate to occur. This assay yields valuable information about MMP isoforms or changes in activation and can be used to analyze the role of MMPs in normal versus pathological conditions.