Titlebook: Breast Cancer; Methods and Protocol Jian Cao Book 2016 Springer Science+Business Media New York 2016 diagnosing breast cancer.clinical prac

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Jian CaoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/190540.jpg

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https://doi.org/10.1007/978-3-642-41769-6strictly defined as invasive carcinomas lacking expression of estrogen receptor, progesterone receptor, and HER2, some use the terms triple-negative and basal-like cancer synonymously. It should be noted that these are not entirely equivalent. Nevertheless, it has been shown that a panel of immunohi

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,Qualitative Analyse von Makromolekülen, for anti-HER2 antibody therapy and should be performed in accordance with current ASCO-CAP guidelines (Hammond et al., J Clin Oncol 29(15):e458, 2011; Wolff et al., J Clin Oncol 31(31):3997–4013, 2013). Here we describe a HER2 FISH methodology to evaluate HER2 gene status in FFPE breast tumor speci

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M. D. Lechner,E. H. Nordmeier,K. Gehrkede of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA s

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M. D. Lechner,E. H. Nordmeier,K. Gehrkesamples, it is very difficult to get methylation profile of only tumor cells. Laser capture Microdissection (LCM) is giving us a chance to isolate the DNA only from the tumor cells without any stroma cell’s DNA contamination. Once we capture the breast tumor cells, we can isolate the genomic DNA whi