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Titlebook: Brassinosteroids; Methods and Protocol Eugenia Russinova,Ana I. Caño-Delgado Book 2017 Springer Science+Business Media LLC 2017 Plant hormo

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楼主: clannish
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Polarized Electrons and Magnetismically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify an Alexa Fluor 647-castasterone (AFCS) from commercially available materials. The reported strategy is also amenable to the introduction of various other amine-based labeling groups.
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Springer Series in Solid-State Sciencescribe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (.) seedlings.
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Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprifferent posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein–protein interactions.
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Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability responding antibodies for the visualization of protein bands. The protocol described has been successfully applied in wild-type, different transgenic, and mutant background plants to study how light alone or in combination with other factors influences protein stability.
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