Titlebook: Brassinosteroids; Methods and Protocol Eugenia Russinova,Ana I. Caño-Delgado Book 2017 Springer Science+Business Media LLC 2017 Plant hormo

难理解

Polarized Electrons and Magnetismass transmembrane domain. The latest updated laboratory protocol is presented as well as examples of data analysis and typical results obtained. Potential drawbacks of the procedure employed for plant membrane proteins will be pointed out.

发怨言

厚颜

Ingenuity

Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues,acts. The protocol is designed for sensitive liquid chromatography-tandem mass spectrometry-based method for simultaneous detection of 22 naturally occurring BRs, including their biosynthetic precursors and most of their biologically active metabolites, without need for derivatization.

诗集

Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Asso interest, allowing tissue-specific immunopurification of the polysome complexes. The methods presented assess establishment and selection of suitable transgenic lines. A protocol for hormonal treatment of the . root as a case study, TRAP and linear amplification of the purified polysome-associated

发誓放弃

Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometrass transmembrane domain. The latest updated laboratory protocol is presented as well as examples of data analysis and typical results obtained. Potential drawbacks of the procedure employed for plant membrane proteins will be pointed out.

引起

The Primary Root of , , (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops,y of the BRASSINOSTEROID INSENSITIVE1-like receptor family in . and its orthologous genes in sorghum. Overall, we support the use of sorghum as a suitable crop model species for the study of BR signaling in root growth and development. The methods presented enable any laboratory worldwide to use sor

Confound

Fulminate

Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Thput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.

Adherent

1064-3745 results.Contains key implementation advice from the expertsThis detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques