Titlebook: Brassinosteroids; Methods and Protocol Eugenia Russinova,Ana I. Caño-Delgado Book 2017 Springer Science+Business Media LLC 2017 Plant hormo

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Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells, of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endom

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Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass SpectrometrSpectrometry/Mass Spectrometry (LCMS/MS) has proven to be a reliable method to qualitatively characterize membrane-bound receptor complexes from plants. Success depends on a range of parameters, such as abundance and stability of the complex and functionality of the tagged receptors, efficiency of t

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Simplified Enrichment of Plasma Membrane Proteins from , Seedlings Using Differential Centrifugatios, including perception of extracellular signals to coordinate cellular changes. Analyses of PM proteins, however, are often limited by the relatively low abundance of these proteins in the total cellular protein pool. Techniques traditionally used for enrichment of PM proteins are time consuming, t

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Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by ImmunoprD INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants

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The Primary Root of , , (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops, plant model system . has yielded important advances in the molecular and cellular understanding of root growth and development. Several studies have uncovered how the hormones brassinosteroids (BRs) control cell cycle and differentiation programs through different cell-specific signaling pathways t

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Springer Series in Solid-State Sciences interest, allowing tissue-specific immunopurification of the polysome complexes. The methods presented assess establishment and selection of suitable transgenic lines. A protocol for hormonal treatment of the . root as a case study, TRAP and linear amplification of the purified polysome-associated