Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

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MAP and TOP Application Case Studies,abeling can be combined with the analysis of gene expression by whole-mount in situ hybridization. This co-immunodetection procedure is well adapted to visualize and quantify the dynamics of de novo neurogenesis. Upon continuous BrdU labeling, the repeated measurements of BrdU-labeling indexes in sp

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Curl-Conformin Basis Functions, cnidarian model organism for studying the evolution of developmental processes. We also provide an overview of existing transgenic . lines that have been used to study conserved and derived aspects of nervous system development.

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Vladimir Leble,George N. Barakosin some of these protocols in detail, with the aim that should prove useful in our much-needed understanding of the origins of bilaterian animals. An anatomical sketch is provided at the beginning as a necessary guide for those not familiar with the Acoela.

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Duje Veic,Marek Kraskowski,Tomasz Bugalskions. Thus, antibody probes that recognize defined tissues, cells, or subcellular structures like axons or synaptic terminals can be used as markers to identify and analyze phenotypes in embryos and larvae. Several antibodies, combined with different labels can be used concurrently to examine protein

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Emilio Di Lorenzo,Simone Manzatodual cells within a labeled NB lineage. These protocols make it feasible to uncover precise lineage relationships between a brain NB and its daughter cells, and to assign gene expression to individual clonal cells. Such lineage-based information is a critical key for understanding the cellular and m

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I. Akkerman,K. Benner,Y. Bazilevsompared to other tissues such as muscles or cuticularized body parts, the interpretation in μCT scans is challenging and needs some practice. The protocol described here is also applicable for larger specimens of a variety of species and spans over 2–3 days resulting in an image stack ready for post

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The Search for Life: Past and Present,nd localization during development. Thus, genetic tools and immunohistochemistry are complementary techniques for studying cellular and developmental processes in .. Protocols for immunostaining in . are similar to those in other organisms; however, some features of these nematodes provide unique ch

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