Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

lutein

书目名称Brain Development影响因子(影响力)




书目名称Brain Development影响因子(影响力)学科排名




书目名称Brain Development网络公开度




书目名称Brain Development网络公开度学科排名




书目名称Brain Development被引频次




书目名称Brain Development被引频次学科排名




书目名称Brain Development年度引用




书目名称Brain Development年度引用学科排名




书目名称Brain Development读者反馈




书目名称Brain Development读者反馈学科排名

galley

厨师

Computational Methods in Applied Sciencesthe fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.

FUSC

毛细血管

CRISPR/Cas9 Genome Editing to Study Nervous System Development in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.

indoctrinate

A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hyeps of cell fate specification to be followed at a single cell resolution. This protocol describes preparation of . embryos, classical in situ hybridization protocol coupled with nuclear staining, and a guide to identify gene expression in specific precursors of the developing brain at neural plate stages of development.

态度暖昧

多样

凝视

Vladimir Leble,George N. Barakosrotocols for wholemount and flat preparations of . embryos, which allow a more detailed analysis of gene expression patterns in relation to the cellular context of the early brain (and facilitate the identification of individual brain neuroblasts) using conventional light microscopy.

MEET

Zhengshun Cheng,Torgeir Moan,Zhen Gaoin multiple colors. This is followed by a detailed protocol as to how to prepare samples for imaging. Finally, we provide specifications to facilitate multichannel image acquisition using confocal microscopy.