Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

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The Search for Life: Past and Present,stages—usually 70–300 μm in size—as well as for vibratome and cryostat sections of complex brains. Although our procedures have been optimized for marine molluscs, they may easily be adapted to other (marine) organisms by the creative neurobiologist.

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Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain, as new model system. The embryonic development of marbled crayfish is well characterized and these animals can be easily cultivated in the lab. This chapter describes protocols for immunolocalization of neuroactive substances in the developing crayfish brain.

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Methods in Brain Development of Molluscsstages—usually 70–300 μm in size—as well as for vibratome and cryostat sections of complex brains. Although our procedures have been optimized for marine molluscs, they may easily be adapted to other (marine) organisms by the creative neurobiologist.

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