Titlebook: Bone Research Protocols; Aymen I.‘Idris Book 20193rd edition Springer Science+Business Media, LLC, part of Springer Nature 2019 osteoclast

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/189709.jpg

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Tumors and Tumor-Like Conditions,osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.

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Handling and Processing of Liver Biopsies,bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.

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90Y Microspheres for Other Liver Metastases,ell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.