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Titlebook: Bone Research Protocols; Aymen I.‘Idris Book 20193rd edition Springer Science+Business Media, LLC, part of Springer Nature 2019 osteoclast

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Liver and Biliary Tract Surgeryon, and resolution of inflammation. Here, we describe three different models mimicking clinic symptoms of human rheumatoid arthritis, which could be transferable to any genetically modified mouse. We will present the protocol for collagen-induced arthritis (CIA), K/BxN serum-induced arthritis (SIA),
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Isolation and Generation of Osteoclastsosteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.
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Isolation and Generation of Osteoblastsbone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.
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