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Titlebook: Bone Research Protocols; Aymen I.‘Idris Book 20193rd edition Springer Science+Business Media, LLC, part of Springer Nature 2019 osteoclast

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期刊全称Bone Research Protocols
影响因子2023Aymen I.‘Idris
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bone Research Protocols;  Aymen I.‘Idris Book 20193rd edition Springer Science+Business Media, LLC, part of Springer Nature 2019 osteoclast
影响因子.The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge .Bone Research Protocols: Methods and Protocols, Third Edition. aims to extensive list of protocols for anyone interested in bone research..
Pindex Book 20193rd edition
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2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studiesell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.
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Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridizationption and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes.
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The Ex Vivo Organ Culture of Boneight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skele
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Ex-Vivo Model Systems of Cancer-Bone Cell Interactionsic and dynamic” culture conditions and their potential use to understand cellular and molecular mechanisms as well as drug testing and discovery. An additional focus of this chapter is to provide details of how to incorporate varying oxygen tension, viz., hypoxic, normoxic, and hyperoxic, in such st
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