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Titlebook: Bone Morphogenetic Proteins; Methods and Protocol Melissa B. Rogers Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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楼主: TEMPO
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Generation and Identification of Genetically Modified Mice for BMP Receptors,modified mice were generated and introduce genotyping methods. These methods include regular PCR and genomic real-time PCR using specific primers based on different constructs in different mice strains.
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https://doi.org/10.1007/978-3-663-01758-5m which utilizes cells transduced with a non-replicating adenovirus containing bone morphogenetic protein 2 (BMP-2) in the E1 region of the cassette. With this method of delivery, small amounts of the protein can incite de novo bone formation.
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Anne Jardin,Karine Broto,Timothée Perdrizeten result in enhanced HO, which can exacerbate rather than improve diagnostic outcomes. As a result of these difficulties, a variety of animal models are needed to study human FOP. Here we describe the methods for creating and characterizing zebrafish conditionally expressing Acvr1l., the first adult zebrafish model for FOP.
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J.-P. Burg,A. Dolati,D. Bernoulli,J. Smitmodified mice were generated and introduce genotyping methods. These methods include regular PCR and genomic real-time PCR using specific primers based on different constructs in different mice strains.
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https://doi.org/10.1007/978-3-319-73942-7n are being made through the use of genetically engineered mouse models that recapitulate the human disease. We describe methods for inducing heterotopic ossification in a mouse model that conditionally expresses the .. allele.
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1064-3745 ls...  Authoritative and cutting-edge, .Bone Morphogenetic Proteins: Methods and Protocols. aims to ensure successful results in the further study of this vital field..978-1-4939-8904-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-3-319-44726-1yeast FLP/. recombination system facilitates experiments to interrogate loss- or gain-of-function for transcription factor activity on known enhancers. This chapter will outline the necessary steps to create the tools needed and conduct somatic cell recombination experiments to interrogate the funct
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