Titlebook: Bone Morphogenetic Proteins; Methods and Protocol Melissa B. Rogers Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Generation and Identification of Genetically Modified Mice for BMP Receptors,een established to study BMP function in development and disease. Mice with altered BMP receptor genes (including global knockout, conditional knockout, and conditional constitutively active transgenic mouse lines) have been particularly informative. In this chapter, we describe how the genetically

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Phenotypic Analyses of Genetically Modified Mice for BMP Receptors, particularly informative for clarifying the role of BMP signaling. In this chapter, we introduce several selected protocols for in vivo functional characterization of BMP receptors in genetically modified mice, including immunohistochemistry of BMP downstream signaling (P-Smad1/5/9 or others), hist

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Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections,bodies to bind to specific antigens. Cellular signaling can be visualized using antibodies raised against phosphorylated proteins. Phosphorylated Smad1, Smad5, and Smad9 are the activated signaling molecules of the BMP pathway that transfer BMP signals from the cell surface to the nucleus. Here we d

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Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds, BMP signaling at various stages of limb development causes defects ranging from complete limb agenesis to oligodactyly, polydactyly, webbing, and chondrodysplasia. To probe the state of BMP signaling in early limb buds, we designed two sets of primers to measure both spatially and quantitatively th

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Pharmacologic Strategies for Assaying BMP Signaling Function,y diverse receptors and ligands whose combinatorial specificity encodes autocrine, paracrine, and endocrine signals essential for regulating tissue growth, differentiation, and survival during embryonic patterning and postnatal tissue remodeling. Aberrant signaling of these receptors and ligands is

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Bone Morphogenetic Proteins (BMPs) and Bone Regeneration,e studies and provides details on the rat femur defect model. This animal model is frequently utilized to measure the efficacy of osteogenic factors like BMP-2. Detailed information about delivery methods, dose range, and dose duration used in BMP-2-related studies are provided.

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1064-3745 tion advice from the experts.This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines,