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Titlebook: Bone Morphogenetic Proteins; Methods and Protocol Melissa B. Rogers Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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发表于 2025-3-21 16:26:16 | 显示全部楼层 |阅读模式
期刊全称Bone Morphogenetic Proteins
期刊简称Methods and Protocol
影响因子2023Melissa B. Rogers
视频video
发行地址Includes cutting-edge methods and protocols.Provide step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bone Morphogenetic Proteins; Methods and Protocol Melissa B. Rogers Book 2019 Springer Science+Business Media, LLC, part of Springer Nature
影响因子.This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls...  Authoritative and cutting-edge, .Bone Morphogenetic Proteins: Methods and Protocols. aims to ensure successful results in the further study of this vital field..
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Cell-Based Gene Therapy System for Delivering BMPs,with accurate posttranslational processing. The use of transduced cells eliminates viral toxicity, allows for targeted expression of the secreted factor at a specific site, and ensures that the therapy will be turned off when the cells are cleared by the organism. Here we describe the delivery syste
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Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing,tificial cDNA overexpression vectors containing either a promoter fragment or specific nucleotide sequence repeats upstream of . luciferase or fluorescent reporters, such as green fluorescence protein (GFP) cDNA. These systems suffer mainly from the lack of chromatin context. Here, we describe the r
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Mutagenesis and Imaging Studies of BMP Signaling Mechanisms in ,,onents and the power of genetic and cell biological approaches applied toward understanding how the pathway signals. In Subheading ., we detail approaches to study the BMP branch of the TGFβ pathway in ..
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Gene Regulation of BMP Ligands in ,ols available. . has three BMP ligands, . (BMP2/4), ., and . (BMP5/6/7/8). Of these genes, the transcriptional regulation of . has been studied, and some of the enhancers directing its spatially specific gene expression have been described. These analyses have used many of the standard tools of mole
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Proteolytic Activation of Bmps: Analysis of Cleavage in , Oocytes and Embryos,that can modulate growth factor activity. Here we provide three protocols that can be used to examine the process of proteolytic activation of Bmps. The first protocol describes how to generate radiolabeled Bmp precursor proteins in . oocytes and then analyze the time course of precursor cleavage by
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