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Titlebook: Biotechnologies for Plant Mutation Breeding; Protocols Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J. Book‘‘‘‘‘‘‘‘ 2017 International A

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Lebensdauerprüfung von Zahnradgetrieben late 1920s. The development of high-throughput and accurate mutation discovery technologies has enabled reverse-genetic screening of mutant populations in the twenty-first century. The majority of mutation-based approaches in crops have involved seed-propagated species. Large bodies of data are ava
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https://doi.org/10.1007/978-3-663-07281-2s. For this reason, they play a very important role in tropical regions’ food security. Several pathogens throughout the tropical areas of the world have become a threat for production, including “black sigatoka” (. Morelet) which is the most damaging disease for . plantations in Cuba. It is present
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https://doi.org/10.1007/978-3-322-88319-3ies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutatio
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https://doi.org/10.1007/978-3-322-88319-3s (TALENs) is provided. As explant for the introduction of TALEN expression constructs, embryogenic pollen cultures are used which consist primarily of haploid cells able to be converted via pollen embryogenesis and genome duplication into fertile doubled haploid plants. Thanks to the haploid nature
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Klaus Birkelbach,Heiner Meulemannties of crop improvement. In the approach described in this chapter, the two units of transcription activator-like effector nucleases (TALENs) required for site-directed cleavage activity are separately used to produce homozygous transgenic lines, each carrying only one unit of the TALEN pair. Cross
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Sebastian Neumeyer,Heiner Meulemannp new varieties of crops and ornamentals. More than 3200 varieties have been produced through mutation induction, and these have contributed towards improving food security in many countries..Proper field techniques can greatly enhance the chances of identifying desirable phenotypes. The protocol de
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