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Titlebook: Biotechnologies for Plant Mutation Breeding; Protocols Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J. Book‘‘‘‘‘‘‘‘ 2017 International A

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发表于 2025-3-21 20:06:34 | 显示全部楼层 |阅读模式
期刊全称Biotechnologies for Plant Mutation Breeding
期刊简称Protocols
影响因子2023Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J.
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发行地址Open Access provided with a grant from the International Atomic Energy Agency.All contributions have been peer-reviewed.An excellent reference source for plant breeders, geneticists and seed companies
图书封面Titlebook: Biotechnologies for Plant Mutation Breeding; Protocols Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J.  Book‘‘‘‘‘‘‘‘ 2017 International A
影响因子.This book is open access under a CC BY-NC 2.5 license..This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species..
Pindex Book‘‘‘‘‘‘‘‘ 2017
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发表于 2025-3-21 23:46:17 | 显示全部楼层
Chemical and Physical Mutagenesis in ,ack of genetic variability hampers the breeding progress, mutagenesis can introduce genetic variation, reveal gene function, and aid in the characterization of candidate genes involved in biological functions. Mutagenized populations are useful for screening for altered phenotypes and physiological
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Creation of a TILLING Population in Barley After Chemical Mutagenesis with Sodium Azide and MNUies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutatio
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Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollens (TALENs) is provided. As explant for the introduction of TALEN expression constructs, embryogenic pollen cultures are used which consist primarily of haploid cells able to be converted via pollen embryogenesis and genome duplication into fertile doubled haploid plants. Thanks to the haploid nature
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Doubled Haploidy as a Tool for Chimaera Dissolution of TALEN-Induced Mutations in Barleyties of crop improvement. In the approach described in this chapter, the two units of transcription activator-like effector nucleases (TALENs) required for site-directed cleavage activity are separately used to produce homozygous transgenic lines, each carrying only one unit of the TALEN pair. Cross
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Field Evaluation of Mutagenized Rice Materialp new varieties of crops and ornamentals. More than 3200 varieties have been produced through mutation induction, and these have contributed towards improving food security in many countries..Proper field techniques can greatly enhance the chances of identifying desirable phenotypes. The protocol de
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Root Phenotyping Pipeline for Cereal Plantsereal plants. The designed pipeline is composed of a drip irrigation system to supplement plants with a medium, a high-resolution root system scanning facility and a method for comprehensive image analysis. The system enables low-effort, accurate and highly repeatable analysis of features of the roo
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