Titlebook: Biotechnologies for Plant Mutation Breeding; Protocols Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J. Book‘‘‘‘‘‘‘‘ 2017 International A

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Optimisation of Somatic Embryogenesis in Cassavaaturation medium (EMM) or can also be treated with mutagenic agents for mutant induction. The resulting primary embryos are recycled three consecutive times, each by fragmentation of the cotyledons followed by culture on embryo initiation medium to produce more matured embryos. Somatic embryos produ

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Creation of a TILLING Population in Barley After Chemical Mutagenesis with Sodium Azide and MNU TILLING population can serve as a renewable resource of mutations for research that uses both forward and reverse genetic approaches. In this chapter, we describe the methods for the development and maintenance of a TILLING population in barley.

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Root Phenotyping Pipeline for Cereal Plantsarameters of the culture medium. This continuous sensing of medium parameters can be applicable for mineral nutrition studies and abiotic stress response testing. The installed drip lines are injected into transparent acrylic tubes (500 mm high, 32/30 mm in outer and inner diameter, with a circular

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Priapism

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A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discoveryelf-extracted single-strand-specific nucleases, typically prepared from crude extracts of celery (., is common for reverse-genetics (e.g. Targeting Induced Local Lesions IN Genomes (TILLING)) and studies of natural nucleotide polymorphisms (e.g. Ecotilling). While protocols have been published descr

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https://doi.org/10.1007/978-3-658-32244-1 thousands of years ago in the domestication of plants and animals. The mechanisms that govern the inheritance of traits were later described by Mendel. In the early decades of the twentieth century, scientists showed that the relatively slow rate of natural mutation could be increased by several or

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