Titlebook: Beta-Arrestins; Methods and Protocol Mark G. H. Scott,Stéphane A. Laporte Book 2019 Springer Science+Business Media, LLC, part of Springer

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enterprise

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Proteomic Analysis of the β-Arrestin Interactomesestin signaling complexes. To investigate changes in the amounts of binding partners under different conditions, we also describe a SILAC (stable isotope labeling by amino acids in cell culture) method to obtain quantitative information for β-arrestin interactomes.

antecedence

Defining Institutional Development (ID)ion, methodology has evolved accordingly over the last three decades since the discovery of the arrestin family. Herein we describe state-of-the-art approaches for studying the role of arrestins (β-arrestin1 . arrestin 2, β-arrestin2 . arrestin 3) in GPCR function in a primary cell type, cultured airway smooth muscle cells.

音乐等

Defining Institutional Development (ID) (BRET)-based techniques can reveal ubiquitination of GPCRs in intact cells and in real time. This chapter describes a step-by-step protocol to evaluate ubiquitination of GPCRs using the BRET methodology.

时代

occult