Titlebook: Beta-Arrestins; Methods and Protocol Mark G. H. Scott,Stéphane A. Laporte Book 2019 Springer Science+Business Media, LLC, part of Springer

Uncultured

MUTE

coddle

intimate

残忍

Institutional Development PerformanceRs). They are now also known to act as multifunctional adaptor proteins binding many non-receptor protein partners to control multiple signalling pathways. β-arrs therefore act as key regulatory hubs at the crossroads of external cell inputs and functional outputs in cellular processes ranging from

Progesterone

wreathe

Kernel

Institutional Development Performanceegration of cellular signalling. We previously found that the angiotensin II (Ang II) type 1 receptor (AT1R) and the prostaglandin F2α (PGF2α) receptor (FP), both important in the control of smooth muscle contractility, form such a functional heterodimeric complex in HEK 293 and vascular smooth musc

标准

Defining Institutional Development (ID) trafficking of internalized GPCRs to lysosomes. Ubiquitination of GPCRs is mediated by specific E3 ubiquitin ligases that are scaffolded by the adaptor proteins called β-arrestins. Traditionally, detection of GPCR ubiquitination is achieved by using ubiquitin antibodies to Western blot immunoprecip

AGOG

https://doi.org/10.1007/978-981-13-7717-4proteins. Here we describe the methods used to identify the interaction sites of arrestin-binding partners on arrestin-3 and the use of monofunctional individual arrestin-3 elements in cells. Our in vitro pull-down assay with purified proteins demonstrates that relatively few elements in arrestin en