Titlebook: Base Excision Repair Pathway; Methods and Protocol Kishor K. Bhakat,Tapas K. Hazra Book 2023 The Editor(s) (if applicable) and The Author(s

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Isolation and Immunodetection of Enzymatic DNA–Protein Crosslinks by RADAR Assayof interest and quantified by normalizing to a DNA loading control. The RADAR assay allows for the detection of specific types of DPCs and the sensitive analysis of the DNA–protein crosslinking activity of various drugs, is adaptable across different cell types and conditions, and requires little specialized equipment.

Cytology

Interactome Profiling of DNA Damage Response (DDR) Mediators with Immunoprecipitation-Mass Spectrometh DDR defects, such as cancer. The protocol described here is a routine PPI analysis procedure that can be performed on samples stimulated with DNA damage. All processes and reagents are optimized for maximum sensitivity on the interactome and minimal contamination for the mass spectrometer.

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Using Affinity Pulldown Assays to Study Protein–Protein Interactions of Human NEIL1 Glycosylase and lycosylase and the checkpoint protein complex RAD9–RAD1–HUS1 (9-1-1). We uncover unique interactions between 9-1-1 and NEIL1, which suggest a possible inhibitory role of the disordered, phosphorylated C-terminal region of RAD9 in regulating NEIL1 activity in base excision repair through lack of physical association of 9-1-1 and NEIL1.

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Introduction: A Meeting in Beirut,lycosylase and the checkpoint protein complex RAD9–RAD1–HUS1 (9-1-1). We uncover unique interactions between 9-1-1 and NEIL1, which suggest a possible inhibitory role of the disordered, phosphorylated C-terminal region of RAD9 in regulating NEIL1 activity in base excision repair through lack of physical association of 9-1-1 and NEIL1.

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https://doi.org/10.1007/978-3-662-11998-3compounds, substrate specificity and cleavage efficiency of repair enzymes, and quantitative structure–function relationships. Overall, the methodology is highly sensitive and can also be modified to explore whether a lesion is processed by NER or NIR activity, as well as to study its miscoding properties in translesion DNA synthesis (TLS).

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