Titlebook: Base Excision Repair Pathway; Methods and Protocol Kishor K. Bhakat,Tapas K. Hazra Book 2023 The Editor(s) (if applicable) and The Author(s

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https://doi.org/10.1007/978-3-662-11998-3ons, bulky DNA adducts, base dimers, base alkylation, cytosine deamination, nitrosation, or other types of base alteration which interfere with DNA replication. Mammalian cells have evolved with a robust defense mechanism to repair these base modifications (damages) to preserve genomic stability. Ba

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https://doi.org/10.1007/978-3-642-49222-8ion of a thermostable polymerase thus resulting in decreased amplification. However, some of the mutagenic DNA base lesions cause little or no distortion in Watson–Crick base pairing. One of the most abundant such lesion is 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo(d)Gua), although it affects the t

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https://doi.org/10.1007/978-3-642-50698-7ch. A critical component of deciphering this repair pathway is developing techniques that detect and quantify specific types of DPCs in cells. Here we describe a protocol for direct detection of enzymatic DPCs from mammalian cells—the RADAR assay. The method involves isolating genomic DNA and DPCs f

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Hebrew Fascism in Palestine, 1922–1942interactions coordinate complex biological processes, such as the DNA damage response (DDR). Induction of DNA damage activates signaling networks where posttranslational modifications cause PPI that facilitate DNA repair and cell cycle coordination. Protein interactome profiling of DDR sensors, tran

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Introduction: A Meeting in Beirut,ins. Pulldown assays capture a target protein fused with an affinity tag and analyze the complexed proteins. Here, we detail methods of pulldown assays for two high-affinity peptide fusion tags, Flag tag (DYKDDDDK) and hexahistidine tag (6xHis), to study protein–protein interactions of human NEIL1 g

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Hebrew Fascism in Palestine, 1922–1942cation) strategy allows rapid isolation of cellular proteins/complexes with a high level of purity. This methodology involves an immuno-affinity-based purification followed by a conformation-based isolation to obtain a highly homogeneous protein/complex. Here, we describe the TAP-mediated isolation

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