Titlebook: Bacterial Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2024Latest edition The Editor(s) (if applicable) and The

刚毅

死亡率

Tractable

,Protein–Protein Interactions: Yeast Two Hybrid,nucleus of yeast. Here, we describe how to use the Matchmaker GAL4-based yeast two-hybrid system to detect the interaction of the . type VI secretion system (T6SS) sheath components TssB and TssC.. The bait and prey gene are expressed as a fusion to the GAL4 DNA-binding domain (DNA-BD) and GAL4 acti

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,Protein–Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid,ing how the machines operate to translocate their substrates. Further, establishing which among the machine components and their substrates interact with each other facilitates (i) advancement in our understanding of the architecture and assembly of the machines, (ii) understanding the substrates’ t

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Bacterial One- and Two-Hybrid Assays to Monitor Transmembrane Helix Interactions,ght also be mediated by helix–helix contacts in the inner membrane. Several assays have been therefore developed to test homotypic and heterotypic interactions between transmembrane α-helices in their native membrane environment. Here, we provide detailed protocols for two genetic assays, TOXCAT and

DAUNT

micronized

,Protein–Protein Interaction: Tandem Affinity Purification in Bacteria,ent proteins of a specific cellular pathway. Deciphering protein–protein interaction networks therefore contributes to a deeper understanding of how cells function. Here we describe the protocol to perform tandem affinity purification (TAP) in bacteria, which enables the identification of the partne

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Laure Journet,Eric CascalesIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180320.jpg

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Bacterial Secretion Systems978-1-0716-3445-5Series ISSN 1064-3745 Series E-ISSN 1940-6029