Titlebook: Bacterial Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2024Latest edition The Editor(s) (if applicable) and The

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Probing Protein Topology and Conformation by Limited Proteolysis, proteases can be used to probe the topology of bacterial membrane proteins. Here, we describe limited protease accessibility assays to define inner membrane protein topology and conformational changes based on digestion profiles.

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,Protein–Protein Interactions: Oxidative Bacterial Two Hybrid,in with an oxidative cytoplasm that promotes correct folding of proteins with disulfide bonds. This genetic background expands the set of host strains suitable for studying protein–protein interactions in vivo by the adenylate cyclase two-hybrid approach.

Palter

,Protein–Protein Interactions: Yeast Two Hybrid,ught into proximity, thus activating transcription of reporter genes. This technology can be widely used to identify interacting partners, confirm suspected interactions, and define interacting domains.

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Tanja Klenk,Frank Nullmeier,Göttrik Wewery in-gel fluorography. Alkyne phospholipids that are the acyl donor in the maturation process of lipoproteins can also be isolated from bacterial membranes for further enzymatic and kinetic studies of acyltransferase that use phospholipids as substrate.

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In-Situ

1064-3745 ation advice from the experts.This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imag

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Thomas Erlenwein,Jürgen Karla,Dennis Maus and drawbacks of each of these approaches are described through many examples of methods that predict secretion, integration into membranes, or subcellular locations in general. The aim of this chapter is to provide a user-level introduction to the field with a minimum of computational theory.

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https://doi.org/10.1007/978-3-658-23668-7ribe a protocol for the purification of outer membranes and inner membranes from .. This allows for compositional analysis of the membranes as well as functional analyses. The procedure can be applied with minor modifications to other bacterial species including those carrying capsular polysaccharide attached to the outer membrane.