Titlebook: Bacterial Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2024Latest edition The Editor(s) (if applicable) and The

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书目名称Bacterial Secretion Systems影响因子(影响力)




书目名称Bacterial Secretion Systems影响因子(影响力)学科排名




书目名称Bacterial Secretion Systems网络公开度




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书目名称Bacterial Secretion Systems被引频次




书目名称Bacterial Secretion Systems被引频次学科排名




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书目名称Bacterial Secretion Systems年度引用学科排名




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书目名称Bacterial Secretion Systems读者反馈学科排名

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Peter K. Neff,Claus Mayer,Uwe Middekegh a French pressure cell (French press) at desired total pressure. Although high-pressure lysis leads to the formation of mostly inverted membrane vesicles (designated and abbreviated usually as ISO vesicles, everted or inverted membrane vesicles (IMVs)), inconclusive results are quite common. This

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https://doi.org/10.1007/978-3-322-83509-3 in . cells expressing the α-fragment of LacZ, LacZα (via the α-complementation phenomenon). The dual nature of the PhoA–LacZα reporter allows a simple way to normalize both enzymatic activities to obtain readily interpretable information about the subcellular location of the fusion site between the

engagement

Peter K. Neff,Claus Mayer,Uwe Middekeybrid proteins retain their activity—and their association state. This chapter describes the principle of the BACTH genetic system and the general procedures to study protein–protein interactions in vivo in ..

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Zivilrechdiche Aspekte des Direct Marketingize the same in vivo can yield quick, advantageous, reliable, and informative protein–protein interactions data. Here, we describe the easily adoptable bimolecular fluorescence complementation and cytology-based two-hybrid assays to understand the bacterial secretions systems.

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https://doi.org/10.1007/978-3-322-94835-9s an example to describe the principle, procedure, and experimental problems of co-IP. First, we show the interaction of two . type VI secretion system (T6SS) sheath components TssB and TssC., and secondly, we show the protocol we used for determining the interaction of an epitope-tagged T6SS effect

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Exploring Uniform, Dual, and Dynamic Topologies of Membrane Proteins by Substituted Cysteine Accesss in proteins, the thiol pKa lies in the range of 8–9, and formation of cysteinyl thiolate ions is optimum in aqueous rather in a nonpolar environment. These features and the ease of specific chemical modification with thiol reagents are central to SCAM™. Membrane side-specific sulfhydryl labeling a