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Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2018 Springer Science+Business Media, LLC 2018 sRNA

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Dietmar Pinkernell,Michael Bargende numbers of sRNA candidates. This chapter outlines a potential workflow for computational sRNA analyses and describes in detail methods for homolog detection, target prediction, and functional characterization based on enrichment analysis. The cyanobacterial sRNA IsaR1 is used as a specific example.
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Motortechnische Grundlagen des Dieselmotorsher promote their degradation or interfere with translation initiation. Because a single sRNA can regulate a considerable number of target mRNAs, we seek to identify those targets rapidly and reliably. Here, we present a robust method based on the co-purification of target mRNAs bound to MS2-tagged
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Direkteinblasung bei Dieselmotorengulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Her
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Instandhaltung von Dieselmotoren,ic physiological behavior of bacterial RNAs. Here we document the step-by-step design and application of two fluorescence-based methods for studying the regulatory interactions RNAs perform in vivo: (i) the in vivo RNA Structural Sensing System (iRS.) for measuring RNA accessibility and (ii) the tri
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