Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2018 Springer Science+Business Media, LLC 2018 sRNA

规范要多

ANTI

https://doi.org/10.1007/978-3-322-90621-2thod comprises a direct labeling of surface proteins on living cells using fluorescent dyes, followed by total protein extraction and subsequent separation of these extracts by 2D gel electrophoresis.

ANIM

https://doi.org/10.1007/978-3-662-67086-6e describe the design of a mutational strategy used to analyze the base pairing between two CU-rich regions of the sRNA Rli22 and the AG-rich Shine-Dalgarno region of the mRNA . in .. The protocol can be employed for mutational studies of base pairing between any sRNA and its mRNA target(s).

喊叫

https://doi.org/10.1007/978-3-658-41781-9thod of ultrapure OMVs and the subsequent extraction of RNA and basic steps of RNA-Seq analysis. Bacterial culture, extracellular supernatant concentration, OMV purification, and the subsequent RNA extraction out of OMVs are described. Specific pitfalls within the protocol and RNA contamination sources are highlighted.

Hyperlipidemia

洞穴

Book 2018ips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Bacterial Regulatory RNA: Methods and Protocols .serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology..

bypass

Book 2018in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements

Ingredient

1064-3745 ation advice from the experts.This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential express

慢慢啃

角斗士