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Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2018 Springer Science+Business Media, LLC 2018 sRNA

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期刊全称Bacterial Regulatory RNA
期刊简称Methods and Protocol
影响因子2023Véronique Arluison,Claudio Valverde
视频videohttp://file.papertrans.cn/181/180319/180319.mp4
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2018 Springer Science+Business Media, LLC 2018 sRNA
影响因子.This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Bacterial Regulatory RNA: Methods and Protocols .serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology..
Pindex Book 2018
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书目名称Bacterial Regulatory RNA影响因子(影响力)




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Host-Pathogen Transcriptomics by Dual RNA-Seqyotic cells under a given condition. However, traditional approaches were unsuitable to measure the abundance of transcripts across kingdoms, which is relevant for biological processes such as bacterial infections of mammalian host cells. This changed with the establishment of “Dual RNA-seq,” which
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Identification of New Bacterial Small RNA Targets Using MS2 Affinity Purification Coupled to RNA Seqher promote their degradation or interfere with translation initiation. Because a single sRNA can regulate a considerable number of target mRNAs, we seek to identify those targets rapidly and reliably. Here, we present a robust method based on the co-purification of target mRNAs bound to MS2-tagged
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Assessment of External Guide Sequences’ (EGS) Efficiency as Inducers of RNase P-Mediated Cleavage ofd as the enzyme that mediates cleavage of precursor tRNAs at the 5′-end termini to generate the mature tRNAs. An important characteristic of RNase P is that its specificity depends on the structure rather than the sequence of the RNA substrate. Any RNA species that interacts with an antisense molecu
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