Titlebook: Bacterial Chemosensing; Methods and Protocol Michael D. Manson Book 2018 Springer Science+Business Media, LLC 2018 Chemoreceptor.Chemotaxis

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Kseniya Katsman,Elizabeth L. Jeglicriety of well-established in vitro methods (Englert et al., Microfluidic techniques for the analysis of bacterial chemotaxis. Methods Mol Biol 571:1–23, 2009). In nature, bacteria are surrounded by heterogeneous chemical gradients; hence, it is essential to understand chemotaxis behavior under such

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https://doi.org/10.1007/978-1-4615-1289-9 even in swimming cells. Bacterial flagellar filaments are long (~10 μm), but of small diameter (~20 nm), and their rotation rates are high (>100 Hz), so visualization is difficult. Dark-field microscopy works well with isolated filaments, but visualization in situ is hampered by light scattered fro