Titlebook: Bacterial Chemosensing; Methods and Protocol Michael D. Manson Book 2018 Springer Science+Business Media, LLC 2018 Chemoreceptor.Chemotaxis

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Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillariesfrom a chemorepellent. Some of these assays use a capillary filled with a chemoeffector/agarose mixture to allow cells to accumulate at the mouth of the capillary. Subsequently, assumptions about the relative strengths of chemotaxis strength are based on visual comparisons. Here, we describe a modif

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Visualizing Chemoattraction of Planktonic Cells to a Biofilmriety of well-established in vitro methods (Englert et al., Microfluidic techniques for the analysis of bacterial chemotaxis. Methods Mol Biol 571:1–23, 2009). In nature, bacteria are surrounded by heterogeneous chemical gradients; hence, it is essential to understand chemotaxis behavior under such

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Labeling Bacterial Flagella with Fluorescent Dyes even in swimming cells. Bacterial flagellar filaments are long (~10 μm), but of small diameter (~20 nm), and their rotation rates are high (>100 Hz), so visualization is difficult. Dark-field microscopy works well with isolated filaments, but visualization in situ is hampered by light scattered fro

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All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins. It is thus a powerful tool to probe structure-function relationships in proteins of interest. In this chapter, we describe how we used all-codon mutagenesis to obtain mutants of the . serine receptor Tsr with amino acid replacements at residue F373, a functionally important site in this protein. W

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In Vitro Assay for Measuring Receptor-Kinase Activity in the , Chemotaxis Pathwayaptor proteins. Attractants and repellents alter the rate of CheA autophosphorylation, either by directly binding the receptors or by indirectly interacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in .. This assay has been

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FRET Analysis of the Chemotaxis Pathway Responsehemotaxis is in gradients of amino acids or sugars, but other physiological stimuli such as pH, osmolarity, redox potentials, and temperature are also known to elicit tactic responses. These multiple environmental stimuli are integrated and processed within a highly sophisticated chemotaxis network